Currently, I am testing out the EpiGnome whole genome bisulfite sequencing kit for the development of sequencing libraries. It is a ‘post-conversion adapter ligation’ kit in which you perform the bisulfite conversion prior to starting the library. In theory this should speed things up while also eliminating the need for methylated adapters (which can survive the bisulfite treatment during library prep through traditional methods). In doing so, the hope would be we would easily synthesize oligos for high multiplexing of WGBS libraries for future work in the lab.
The kit has been run on seven Brachypodium distachyon samples at the moment and there appears to be product out the other end. I will be performing qPCR quantification and sample pooling for downstream cluster generation at our sequencing facility here at the ANU